Journal: Cancer Science

Article Title: Design and Evaluation of Eb 4 Mab ‐7‐ mG 2a : A Dual‐Action Anti‐ EphB4 Monoclonal Antibody for Targeted Breast Cancer Therapy

doi: 10.1111/cas.70198

Figure Lengend Snippet: Prognostic significance of EphB4 expression in breast cancer and establishment of Eb 4 Mab‐7‐mG 2a . (A) Kaplan–Meier survival analysis of OS in breast cancer patients based on the expression levels of EphB family genes (EphB1, EphB2, EphB3, EphB4, and EphB6) using RNA‐seq data from KMplot. Among these genes, high expression of EphB2 and EphB4 was significantly associated with poor prognosis (log‐rank p < 0.05). Patients were then stratified into high and low expression groups using the optimal cutoff within the interquartile range. (B) Generation of a mouse IgG 2a version of the anti‐EphB4 monoclonal antibody (Eb 4 Mab‐7‐mG 2a ). The V H and V L regions of B4Mab‐7 (mouse IgG 1 ) were cloned into expression vectors containing mouse IgG 2a C H and C L regions. These vectors were transfected into BINDS‐09 cells to produce Eb 4 Mab‐7‐mG 2a . (C) Left: Expression of EphB4 and β‐Actin in CHO‐K1, CHO/EphB4, MCF‐7, and EphB4‐KO MCF‐7 cells. Right: Expression of EphB4 and GAPDH in normal human adult breast tissues, MCF10A (non‐tumorigenic mammary epithelial cells), MCF‐7, BT‐474, SK‐BR‐3, MDA‐MB‐468, and HBC5 cells.

Article Snippet: Aside from cell lysates, a commercially available normal human breast tissue lysate (Total Protein–Human Adult Normal Tissue: Breast, Novus Biologicals, Littleton, CO, USA) was used to assess expression in normal breast tissue.

Techniques: Expressing, RNA Sequencing, Clone Assay, Transfection

Journal: Cancer Medicine

Article Title: Upregulated IQUB promotes cell proliferation and migration via activating Akt/ GSK 3β/β‐catenin signaling pathway in breast cancer

doi: 10.1002/cam4.1568

Figure Lengend Snippet: IQUB is significantly upregulated in human breast cancer tissues and cells. A, IQUB protein expression (the brown staining areas) was increased in breast cancer, which was detected in 110 cases of human breast cancer tissue microarray by immunohistochemistry. B, The expression of IQUB in poor differentiation of breast cancer tissues was higher than that in well differentiation of breast cancer tissues. C, IQUB mRNA expression was upregulated in breast cancer tissues (16/20) than paired normal breast tissues which was analyzed by RT ‐ qPCR ( x ‐axis represent 20 pairs of tissue samples). D, IQUB mRNA and protein expression were detected by RT ‐ qPCR and Western blotting in normal breast cell line MCF ‐10A and breast cancer cell lines MCF ‐7 and MDA ‐ MB ‐231. Values represent the mean ± SD from 3 independent measurements. ** P < .01, *** P < .001

Article Snippet: The human breast cancer tissue microarray (the TMA ID: BC081120a) was purchased from Alenabio company (Xi'an, China) which contains 110 cases of breast cancer tissues and corresponding paracancerous tissues.

Techniques: Expressing, Staining, Microarray, Immunohistochemistry, Quantitative RT-PCR, Western Blot

Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Survivin 2α expression (relative to normal tissue).

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing

Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Expression of survivin splice variants in medulloblastoma (relative to survivin)

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing